flow-cytometry analysis pipeline Search Results


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Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell <t>transcriptome</t> sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.
Precise Whole Transcriptome Assay Analysis Pipeline V2.0, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell <t>transcriptome</t> sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.
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Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell <t>transcriptome</t> sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.
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Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell <t>transcriptome</t> sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.
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Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell <t>transcriptome</t> sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.
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Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell <t>transcriptome</t> sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.
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Image Search Results


Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell transcriptome sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.

Journal: Frontiers in Immunology

Article Title: Thymic epithelial cell fate and potency in early organogenesis assessed by single cell transcriptional and functional analysis

doi: 10.3389/fimmu.2023.1202163

Figure Lengend Snippet: Heterogeneity of EPCAM + PLET1 + cells in the E10.5 and E12.5 thymic primordium. (A) Schematic showing experimental design. Thymic primordia were microdissected from E10.5 and E12.5 wild-type embryos and dissociated to single cell suspensions. Single E10.5 and E12.5 EPCAM + PLET1 + TEC were then deposited into BD Precise WTA Single Cell Encoding plates by flow cytometry processed for single cell transcriptome sequencing using BD Precise WTA reagents. (B, C) tSNE visualizing the clustering results with Seurat colored by cluster (B) and developmental stage (C) (n=709 cells). (D) Heat map showing differentially expressed genes among the eight clusters. (E) Expression of Notch signalling related plus selected other genes across all clusters. (F) Violin plot showing expression of selected markers across all clusters.

Article Snippet: Pre-processing of the FASTQ files was performed using the BD: Precise Whole Transcriptome Assay Analysis Pipeline v2.0.

Techniques: Flow Cytometry, Sequencing, Expressing